Two people have died following a collision on the A38 north of junction 1 of the M50 on Friday.
The incident, which involved a blue Volkswagen Golf and a horse-and-trap, happened at around 11.35pm.
Two men, both from Worcestershire, who were riding in the trap, and the horse were declared dead at the scene. The coroner has been informed.
No other people were believed to have been injured in the collision.
Closures remain in place at the junction and towards Upton while a full investigation into the circumstances is carried out.
Anybody who may have been in the area at the time is being asked to come forward and speak to police.
Anyone who witnessed the collision or who has relevant dashcam footage is asked to contact Gloucestershire Constabulary through the website, quoting incident 563 of 15 September: https://www.gloucestershire.police.uk/contact/af/contact-us/us/a-matter-involving-driving-vehicles-or-a-road-traffic-incident/